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21-23 March 2018

Speakers


Barbara Gerten, Merck
Prof Dr Diana Graubaum, Beuth University of Applied Sciences
Alexander Bartes, Roche Diagnostics
Hilke Hubert, Bruker UK Ltd
Martina Heldt, Beuth University of Applied Sciences
Beate Rücker, Beuth University of Applied Sciences



Learning Objectives


You will learn how to recognise microbiological cultures. You will further get to know classical procedures for the selection and identification and how to analyse the microbiological concept. In addition you will get an insight into the needed media and in modern rapid methods.

Due to the limited number of participants and the supervision by experienced lab staff you will be able to do the work, to employ the methods and to make the analyses yourself. For that purpose there will be lab places with microscopes in the S2 lab for every participant. Accompanying presentations and a comprehensive documentation will provide the necessary knowledge base.

Background


Microbiological trainings are commonly based on presentations or theoretical workshops. Quite frequently there is either no advanced practical training at all or it is only very limited.

This completely new designed lab course is supposed to be an extension of the microbiological seminars as part of the GMP Certification Programme “Microbiological Laboratory Manager”.

Target Group


Personnel from labs for
  • Quality Control
  • Hygiene
  • Monitoring
  • with basic microbiological knowledge.
Special thanks for supporting this course goes to BioMérieux, Bruker, Merck, Roche Diagnostics and Dr. Weigert GmbH.

Programme


Wednesday 21 March 2018, 09.30-18.00 h
  • Welcome and organisational issues
  • Basic knowledge identification of microorganisms
  • Handling and examination of prepared cultures (different samples e.g. environmental monitoring, water on contact plates, swabs, membranes)
  • Preparation of pure cultures
  • Introduction into the work with microscope and identification of microorganisms including staining procedures
  • Practice of biochemical rapid tests
Thursday, 22 March 2018, 08.30-18.00 h
  • Overview Molecular genetic methods and Mycoplasma detection
  • Identification with Maldi-TOF MS
  • Practice in manual biochemical identification methods (Multi-Test-Systems, additional tests and special media, e.g. chromogenic media using the prepared cultures
  • Practice in molecular genetic rapid methods using the example of Mycoplasma detection
  • Practice in identification of bacteria, yeast and moulds by MALDI-TOF MS using the prepared cultures
Friday, 23 March 2018, 08.00-15.30 h
  • Analysis of the results from molecular genetic rapid methods, MALDI-TOF MS and manual biochemical methods
  • Consolidation and discussion of all results from bacterial identification
  • Basic knowledge and practice of yeast and moulds identification including microscopy
  • Beneficial literature and helpful addresses